How to Properly Clean and Use Your Centrifuge?

A centrifuge is one of the most critical pieces of equipment in any laboratory, ensuring efficient separation of substances based on density. However, improper use or neglecting routine maintenance can lead to contamination, equipment failure, or even hazardous situations.

There are many types of centrifuges in the market, each designed for specific applications, including fixed-angle, swing-out rotor, ultracentrifuges, and microcentrifuges. Choosing the right centrifuge and maintaining it properly is essential for achieving accurate and reliable results.

In this guide, we’ll walk you through how to properly clean and use your centrifuge to ensure longevity, accuracy, and safety in your lab work. Follow these best practices to maintain your equipment in optimal condition.

Why Proper Cleaning and Usage Matter?

Before diving into the step-by-step process, it’s essential to understand why cleaning and proper usage are crucial:

  • Prevents cross-contamination
  • Extends the lifespan of your centrifuge
  • Ensures accurate and reliable test results
  • Reduces the risk of hazardous spills or damage
  • Maintains compliance with lab safety regulations

Ignoring proper maintenance can lead to costly repairs and compromised results. Let's ensure your centrifuge stays in peak condition!

Step-by-Step Guide: How to Use a Centrifuge Correctly?

Read the Manufacturer’s Instructions

Every centrifuge model is different. Before using your centrifuge, always review the user manual provided by the manufacturer. This ensures you understand the correct operating procedure, speed limits, and weight balance guidelines.

Inspect the Centrifuge Before Use

Before every run:

  • Check for cracks or damage in the rotor and tubes.
  • Ensure the centrifuge is placed on a stable, level surface.
  • Verify that the lid and safety lock are working properly.

Load Samples Properly

  • Always balance tubes with equal volume and weight to prevent vibrations.
  • Use matched sets of tubes and avoid overfilling.
  • Secure the rotor lid before starting the centrifuge.

Set the Correct Speed and Time

  • Refer to your experiment protocol and centrifuge manual to select the appropriate RPM (Revolutions Per Minute) or RCF (Relative Centrifugal Force).
  • If you're unsure about RPM vs. RCF, check out our guide: Understanding RPM and RCF

Never Open the Lid While Spinning

  • Wait until the centrifuge fully stops before opening.
  • Sudden interruptions can damage the machine and pose safety risks.

How to Properly Clean a Centrifuge?

Cleaning your centrifuge regularly prevents contamination, corrosion, and sample degradation. Follow these steps for optimal hygiene and maintenance.

Daily Cleaning Routine

  1.           Unplug the centrifuge before cleaning.
  2.       Wipe down the exterior with a mild detergent or ethanol solution.
  3.       Remove any dust, spills, or residue from the chamber.

Cleaning the Rotor

  1.           Remove the rotor and wash it with a neutral pH detergent and warm water.
  2.       For stubborn stains, soak in a 10% bleach solution, then rinse thoroughly.
  3.       Dry the rotor completely before reinstalling it.

Disinfecting After Biohazardous Samples

  1.          If working with biohazardous materials, disinfect with a 70% isopropyl alcohol solution.
  2.       Ensure proper disposal of contaminated waste following lab safety regulations.

Preventing Corrosion and Wear

  1.        Avoid using harsh chemicals that can degrade materials.
  2.       Apply a light coat of lubricant to metal parts if recommended by the manufacturer.

Common Centrifuge Mistakes & How to Avoid Them

  • Overloading the centrifuge: Can cause imbalance and damage the rotor.
  • Using the wrong rotor type: Different applications require fixed-angle or swing-out rotors.
  • Learn the difference: Fixed vs Swing-Out Rotor for Centrifuge
  • Not cleaning regularly: Leads to contamination and corrosion over time.
  • Ignoring unusual noises: Always investigate strange sounds or vibrations to prevent failures.

When to Service Your Centrifuge?

Even with regular maintenance, professional servicing is required periodically. Consider scheduling a check-up if:

  • You notice vibrations or loud noises during operation. 
  • The rotor appears damaged or worn out.
  • The centrifuge fails to reach set RPM/RCF.
  • There are persistent error messages or malfunctions.

For professional maintenance, check out our guide: Laboratory Centrifuge: The Basics of Operation & Maintenance.

 

Looking for a High-Performance Centrifuge? FC-BIOS Has the Perfect Solution!

At FC-BIOS, we specialize in top-quality centrifuge systems engineered for accuracy, reliability, and long-term performance. Whether you need a new centrifuge, expert maintenance, or replacement parts, our dedicated team is here to support you.

Browse our centrifuge selection today! Need help choosing the right centrifuge? Read: How to Select the Right Centrifuge for Your Application.

Keep your lab running efficiently with FC-BIOS – Your Trusted Partner in Laboratory Equipment!